Differential centrifugation, a technique intro­duced to cellular study from the early forties through the mentioned biologist and Nobel laureate Albert Claude, has become the basic processes for isolating subcellular particles and entails the stepwise removal of lessons of particles by successive centrifugations at increas­ing RCF.

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Instead, the particles will sediment with the gradient until eventually they reach their isodense or isopycnic placement.

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The microsomal supernatant is often called the soluble stage on the cells, or cytosol, and involves soluble proteins, soluble nucleic acids, soluble polysac­charides, lipid droplets, together with other minute particles. During the process just described the liver tissue is sepa­rated into four significant fractions.